Comparison of phase-contrast and fluorescence digital autofocus for scanning microscopy
نویسندگان
چکیده
منابع مشابه
Digital autofocus methods for automated microscopy.
Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with multifield scanning are detailed.
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ژورنال
عنوان ژورنال: Cytometry
سال: 1994
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990160402